gntP [2018-02-12T15:07:13.000Z]

gluconate permease

locus: BSU40070

pI: 8.10

mw: 46.49 kDa

protein length: 448 aa

gene length: 1344 bp

function: gluconate uptake

product: gluconate permease

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.3|Carbohydrate transporter]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.13|Utilization of gluconate]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,115,711 → 4,117,057

The protein

Protein family

• gntP permease family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|C62164C159495FDE001F36AF5F6E35C10812ABE4|YojA]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|gntR]-[gene|72404FA6ECD62AEA3EFDB934DFB8DA581875605C|gntK]-[gene|C6810A4ACD51EB8D14E5B30163348058196266A1|gntP]-[gene|7E7187AA2DC172018A63A7B9CE48124F30311639|gntZ]
  description: [pubmed|10746760,3020045]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3020045], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|GntR]: repression, [Pubmed|3020045], in [regulon|1C566E54F8EF1A17A365FC49DFDA452AC5D0CA64|GntR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by gluconate ([protein|search|GntR]) [Pubmed|3020045]

Biological materials

Mutant

• BKE40070 (Δ[gene|C6810A4ACD51EB8D14E5B30163348058196266A1|gntP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAATGCCCTCCCCTT, downstream forward: _UP4_TGAAATTAAGAAGGAGCTGT
• BKK40070 (Δ[gene|C6810A4ACD51EB8D14E5B30163348058196266A1|gntP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAATGCCCTCCCCTT, downstream forward: _UP4_TGAAATTAAGAAGGAGCTGT

References

• 3011959,8288545,3037520,2537826,3020045,10746760,8370661,1659648,220817675